Dual-Glo® Luciferase Assay System Technical Manual

Literature # TM058

The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells containing genes for firefly and Renilla luciferases, grown in 96- or 384-well plates. The Dual-Glo® Luciferase Reagent can be added directly to cells in growth medium without washing or preconditioning. This reagent induces cell lysis and acts as a substrate for firefly luciferase, producing a stable luminescent signal that can be read over a period of two hours, with >60% of the luminescent signal retained. Addition of Dual-Glo® Stop & Glo® Reagent quenches the luminescence from the firefly reaction by at least 10,000-fold and provides a substrate for Renilla luciferase in a reaction that can also be read within 2 hours (with similar signal retention). The Dual-Glo® Luciferase Assay System is designed to work in growth media commonly used for mammalian cells with or without added serum.