AMP-Glo™ Assay Technical Manual

Literature # TM384

The AMP-Glo™ Assay is a homogeneous assay that generates light signal from any reaction that produces AMP as a reaction product. This versatile system can assay multiple types of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP. The AMP-Glo™ Assay is designed to quantitatively monitor the concentration of AMP in a biochemical reaction in a high-throughput format. The stable luminescent signal of the assay eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate.