cAMP-Glo™ Max Assay Technical Manual

Literature # TM347

The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate G protein-coupled receptors (GPCRs) coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo™ Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on GPCRs.

The cAMP-Glo™ Max Assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity; this stimulation depletes ATP in the reaction resulting in decreased light production in a coupled luciferase reaction.