Promega Corporation

GoTaq® 1-Step RT-qPCR System Technical Manual

  • Instructions for Use of Product(s)
  • A6020

Literature # TM355

GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The GoTaq® 1-Step RT-qPCR System combines the benefits of GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix for efficient,sensitive and linear one-step RT-qPCR quantification over a wide range of RNA template inputs.

The GoTaq® 1-Step RT-qPCR System contains the proprietary fluorescent DNA-binding dye, BRYT Green® dye, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA), than does SYBR® Green I.

Printed in USA 8/11.

PDF Icon Complete Protocol PDF (648kb)

PDF Icon Quick Protocol PDF (199kb)

  • Warning
  • It appears that you have Javascript disabled. Our website requires Javascript to function correctly. Please enable Javascript to experience our website.

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.

Click Here for Technical Questions and Ordering Support »