Promega Corporation

PowerPlex® 21 System Technical Manual

Literature # TMD034

The PowerPlex® 21 System allows co-amplification and four-color fluorescent detection of 21 loci (20 STR loci and Amelogenin), including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA. The PowerPlex® 21 System provides all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. This manual contains a protocol for use of the PowerPlex® 21 System with the GeneAmp® PCR System 9700 thermal cycler in addition to protocols to separate amplified products and detect separated material. The system is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Printed in USA. Revised 7/12.

Language:

PDF Icon Complete Protocol PDF (15103kb)

  • Warning
  • It appears that you have Javascript disabled. Our website requires Javascript to function correctly. Please enable Javascript to experience our website.

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.

Click Here for Technical Questions and Ordering Support »