PowerPlex® Y System Technical Manual

Literature # TMD018

The PowerPlex® Y System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing, and tissue culture strain identification. This system allows co-amplification and three-color detection of twelve loci. The system contains the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL); one primer specific for DYS385a/b, DYS390 and DYS393 is labeled with carboxy-tetramethylrhodamine (TMR); and one primer specific for DYS19, DYS392, DYS437 and DYS438 is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein (JOE). The PowerPlex^® Y System is designed specifically for use with the ABI PRISM® 310 Genetic Analyzer and is compatible with the ABI PRISM® 377 DNA Sequencer and the ABI PRISM® 3100 Genetic Analyzer.