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Assay Virtually Any Kinase with Kinase-Glo® Plus Luminescent Kinase Assay: A...

Assay Virtually Any Kinase with Kinase-Glo® Plus Luminescent Kinase Assay: A Homogeneous High-Throughput Assay

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Abstract

Phosphotransferases represent a major group of cellular enzymes that use ATP as a substrate and are implicated in a wide variety of cellular functions including proliferation, differentiation and apoptosis. Their substances encompass a wide range of chemical structures including proteins, peptides, sugars and lipids. Because of the significance of these enzymes in cellular functions, they have become valid targets for drug development. The Kinase-Glo® Plus Assay can be used to monitor the activity of kinases and screen for kinase inhibitors. This homogeneous, non-radioactive assay is robust and amenable to high-throughput applications.

Cell Notes 12, 8–12.

Said Goueli and Kevin Hsaio

Promega Corporation
Publication Date: 2005

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