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AgarACE™ Agarose-Digesting Enzyme Part Three: DNA/RNA Recovery and Applications Testing...

AgarACE™ Agarose-Digesting Enzyme Part Three: DNA/RNA Recovery and Applications Testing for Use in Translation Transcription/Translation Prime-a-Gene® Labeling and Fluorescent DNA Sequencing

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Abstract

AgarACE™ Enzyme is a unique agarase that enables simple, quantitative recovery of nucleic acids from agarose gels. Previously, we presented data describing the use of AgarACE™ Enzyme in cloning, sequencing and PCR-related applications. In this article, we present data on recovery of supercoiled plasmid DNA, high molecular weight linear DNA and RNA, and show that DNA and RNA isolated using AgarACE™ Enzyme perform well in four additional popular applications.

Promega Notes 61, 21.

Mark Knuth, Kimberly Knoche and Paul Otto

Promega Corporation
Publication Date: 1997

Download Article (316 KB)

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