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Separate Isolation of Genomic DNA and Total RNA from Single Samples Using the SV Total...

Separate Isolation of Genomic DNA and Total RNA from Single Samples Using the SV Total RNA Isolation System

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Abstract

Promega's SV Total RNA Isolation System provides a fast and simple technique for the purification of intact total RNA from tissues, cultured cells and whole blood samples. We describe here a modification of the protocol that provides a rapid and safe method for the sequential purification of DNA and RNA from the same sample of tissue, cultured cells or whole blood, using Promega's SV Total RNA Isolation System. A simple DNase step minimizes genomic DNA carryover from the purified RNA when only RNA is desired. The method also allows the purification of only genomic DNA, when this is desired. The nucleic acids isolated using this method are of high purity and are ideally suited for applications such as cDNA cloning, RT-PCR, PCR, Northern and Southern blots and RNase protection assays. With a minor modification, this protocol may also be used for the isolation of hepatitis C virus (HCV) RNA suitable for RT-PCR.

Promega Notes 69, 19.

Paul Otto1, Dan Kephart1, Rex Bitner1, Suzanne Huber2 and Karl Volkerding2

1Promega Corporation; 2Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison

Publication Date: 1998

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