Promega Corporation

CellTiter-Glo® Luminescent Cell Viability Assay: Fast Sensitive and Flexible

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Abstract

Promega's CellTiter-Glo™ Luminescent Cell Viability Assay is a highly sensitive method for assaying cell proliferation and cytotoxicity. The assay uses a unique, stable form of luciferase to measure ATP as an indicator of viable cells. The luminescent signal produced is proportional to the number of viable cells present in culture. The homogeneous format of the assay is well suited for high-throughput applications and is scalable from 96-, 384- and 1536-well formats with little or no loss in sensitivity. Results can be achieved rapidly, while the extended glow luminescence feature offers flexibility in processing large batches of plates.

Promega Notes 81, 2–5.

Terry Riss1, Rich Moravec1, Michael Beck1, Rita Hannah1, Karen Wilson2 and Robert Swanson3

1Promega Corporation, 2Trenton College of New Jersey, and 3Pharmacopeia, Inc.

Publication Date: 2002

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