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Luminogenic Enzyme Substrates: The Basis for a New Paradigm in Assay Design

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Abstract

Luciferin derivatives as luminogenic substrates provide the basis for a new paradigm in enzyme assay design that brings the advantages of bioluminescence—superior sensitivity, resistance to interference, and ease of use—to the enzymology researcher. Here we highlight our wide selection of bioluminescent enzyme substrates for proteases, metabolic enzymes and ß-galactosidase.

Promega Notes 100, 22–26.

Poncho L. Meisenheimer1, Martha A. O’Brien2, and James J. Cali2

1Promega Biosciences, Inc., 2Promega Corporation

Publication Date: 2008

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