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A Luminescent Cytotoxicity Assay for Drug Discovery Scientific Poster

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Abstract

A. Niles, K. Rashka, D. Lange, B. McNamara, P. Guthmiller, T. Worzella, M. Scurria1 L. Bernad1, T.L. Riss
Promega Corporation, Madison, WI 53711 and 1Promega Biosciences, San Luis Obispo, CA 93401

We have developed a non-lytic, homogeneous, luminescent assay method that embodies a number of features useful for cytotoxicity testing in high density multi-well plate formats. This assay measures a ubiquitous and conserved proteolytic activity corresponding with loss of cellular membrane integrity. The assay demonstrates superior performance with respect to sensitivity and useful linear range of cell numbers when compared with other commercially available luminescent cytotoxicity assays. The assay chemistry also demonstrates an improvement in marker half-life and resulting luminescent signal stability. Lastly, the assay can be conveniently coupled with a fluorescent measure of viability to multiplex viability and cytotoxicity assays which offers additional utility for response normalization and determination of compound specific mode-of-action.

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  • Part# PS053
  • Printed in USA.

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