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J. Forensic Sci. 52, 1073–6. Characterization of the variant allele 9.2 of Penta D locus. 2007

Miozzo, M.C., Maxzud, M.K., Pacharoni, C.M., Mutal, S.A. and Modesti, N.M.

Notes: During casework analysis using the PowerPlex® 16 System, the authors identified an off-ladder allele at the Penta D locus as the microvariant allele 9.2. DNA from individuals with the 9.2 allele was amplified using a single primer pair specific for Penta D, and the amplification products were purified and sequenced to characterize the microvariant allele. PCR products were purified using the Wizard® PCR Preps DNA Purification System. Sequence analysis revealed that the 9.2 allele has 10 STR repeats and a TAA deletion in the 3´ flanking region. (3771)

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J. Leukoc. Biol. 79, 202–13. Induction of intracellular calcium elevation by {Delta}9-tetrahydrocannabinol in T cells involves TRPC1 channels. 2006

Rao, G.K. and Kaminski, N.E.

Notes: The authors studied the relationship between transient receptor potential canonical (TRPC) channels and Ca2+ elevation in the cannabinoid-2 receptor-expressing human peripheral blood-acute lymphoid leukemia (HPB-ALL) human T cell line. Total RNA from HPB-ALL cells was subjected to RT-PCR and the bands for TRPC1 were excised from a 1.2% NuSieve 3:1 agarose gel, purified using the Wizard® PCR Preps DNA Purification System and sequenced. Using 20nM synthesized siRNA specific for TRPC1 and a nonsilencing control sequence, 2.5 × 105 HPB-ALL cells/ml (a human T cell line) were transiently transfected for 48 hours using the CodeBreaker™ siRNA Transfection Reagent. After this 48-hour incubation, the siRNA-treated cells were either used for calcium determination or harvested and washed, and the RNA was isolated using the SV Total RNA Isolation System. The RNA was used for quantitative real-time PCR. (3316)

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J. Virol. 78, 9936-9946. Poly(ADP-Ribose) polymerase 1 binds to Kaposi's sarcoma-associated Herpesvirus (KSHV) terminal repeat sequence and modulates KSHV replication in latency. 2005

Ohsaki, E., Ueda, K., Sakakibara, S., Do, E., Yada, K. and Yamanishi, K.

Notes: Three terminal repeat sequences (TR) from Kaposi's sarcoma-associated herpesvirus (KSHV) were PCR amplified and then purified using the Wizard® PCR Preps DNA Purification System. The PCR products were 386, 424, and 307 base pairs in size.  These TR products were used in PARP1-DNA ELISA assays.  The authors also used the Wizard® SV Genomic DNA Purification System to isolate DNA from a BC3 cell line infected with KSHV. The isolated DNA was used in real-time PCR to assess KSHV copy number in cells. (3232)

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J. Clin. Invest. 109, 923-930. The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo 2002

Kemp, E.H., Waterman, E.A., Hawes, B.E., O'Neill, K., Gottumukkala, R.V.S.R.K., Gawkrodger, D.J., Weetman, A.P., and Watson, P.F.

Notes: T4 Polynucleotide Kinase was used to phosphorylate primers before ligating them into a phagemid vector to allow cloning of Sfi I-restricted DNA fragments. Phagemid was prepared from XL1-Blue cells using the Wizard® Minipreps DNA Purification System. Inserts were PCR amplfied from the phagemid and purified using the Wizard® PCR Preps DNA Purification System.  In separate experiments, the cDNA for the melanin concentrating hormone receptor 1 cDNA was cloned from total melanocyte RNA using MMLV Reverse Transcriptase. In vitro translation of the cDNA was performed using the TnT T7 Coupled Reticulocyte Lysate System and Canine Pancreatic Microsomal Membranes. (2602)

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Infect. Immun. 68, 7180-7185. Phylogeny of Vibrio cholerae based on recA sequences. 2000

Stine, O.C., Sozhamannan, S., Gou, Q., Zheng, S., Morris, J.G., Jr., and Johnson, J.A.

Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

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J. Biol. Chem. 274, 17257-17266.. Divalent cations differentially regulate integrin alpha II b cyotplasmic tail binding to beta 3 and to calcium- and integrin-binding protein 1999

Vallar, L., Melchior, C., Plançon, S., Drobecq, H., Lippens, G., Regnault, V., Kieffer, N.

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and intergrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

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Am. J. Bot. 86, 1168-1181. Phylogeny and evolution of the Betulaceae as inferred from DNA sequences, morphology and paleobotany. 1999

Chen, Z.D., Manchester, S.R., Sun, H.Y.

Notes: PCR products were purified with the Wizard® PCR Preps DNA Purification System and directly sequenced with the SILVER SEQUENCE™ DNA Sequencing System. (1334)

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J. Biol. Chem. 274, 19644-19648. Specific DNA recognition by F factor TraY involves beta-sheet residues. 1999

Lum, P.L., Schildbach, J.F.

Notes: Plasmids were purified with either the Wizard® Plus Minipreps DNA Purification System or the Wizard® Plus Midipreps DNA Purifications System. PCR products were purified with the Wizard® PCR Preps System. (0751)

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Am. J. Hum. Genet. 62, 70-76. A presenilin-1 truncating mutation is present in two cases with autopsy- confirmed early-onset Alzheimer disease. 1998

Tysoe, C., Whittaker, J., Xuereb, J., Cairns, N.J , Cruts, M., Van Broeckhoven, C., Wilcock, G., Rubinsztein, D.C.

Notes: PCR products were purified by Wizard® PCR Preps DNA Purification System and sequenced by use of the fmol® DNA Cycle Sequencing Systems. (0247)

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Plant Cell 10, 297-308. a-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica. 1998

Yamamoto, E., Zeng, L. and Baird, W.

Notes: The pGEM® -T Easy Vector Systems, Wizard® PCR Preps DNA Purification System and Wizard® Plus Minipreps DNA Purification Systems were used in this study. (1959)

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Am. J. Hum. Genet. 63, 1025-1035. An analysis of phenotypic variation in the familial cancer syndrome von Hippel-Lindau disease: evidence for modifier effects. 1998

Webster, A. R. , Richards, F. M. , MacRonald, F. E. , Moore, A. T. , Maher, E. R.

Notes: PCR was used to amplify the VHL coding and promoter region in six fragments from genomic DNA, and SSCP analysis was performed to detect intragenic mutations. Fragments producing an aberrant SSCP band were purified using the Wizard® PCR Preps DNA Purification System and subsequentially sequenced. (0176)

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Genetics 149, 1081-1088. Gene silencing by DNA methylation and dual inheritance in Chinese hamster ovary cells. 1998

Paulin, R. P., Ho, T., Balzer, H. J., Holliday, R.

Notes: Bisulphite-treated DNA was purified using the Wizard® DNA Clean-Up System prior to PCR. The amplified DNA was then cleaned using a Wizard® PCR Preps DNA Purification System prior to cloning and the cloned DNAs were sequenced using the fmol® DNA Cycle Sequencing System. (0001)

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J. Biol. Chem. 273, 26426-26431. Genetic probing of the stalk segments associated with M2 and M3 of the plasma membrane H+-ATPase from Saccharomyces cerevisiae. 1998

Soteropoulos, P., Perlin, D.S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from 3ml S. cerevisiae cultures grown 18-20 hours in YPD broth. The isolated DNA was used for PCR amplification of a 3.5kb seqment. The amplimer was purified using the Wizard® PCR Preps System. (0356)

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Am. J. Hum. Genet. 63, 360-369. Human propionyl-CoA carboxylase beta subunit gene: exon-intron definition and mutation spectrum in Spanish and Latin American propionic acidemia patients. 1998

Rodriguez-Pombo, P., Hoenicka, J., Muro, S., Perez, B., Perez-Cerda, C., Richard, E., Desviat, L. R. and Ugarte, M.

Notes: Wizard® PCR Preps DNA Purification System was used to purify PCR products. 100 ng of the PCR products was sequenced using the fmol® DNA Cycle Sequencing System. (0488)

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Proc. Natl. Acad. Sci. USA 95, 8613-8618. Localization of ADP-riboxylation factor domain protein 1 (ARD1) in lysosomes and golgi apparatus 1998

Vitale, N., Horiba, K., Ferrans, V.J., Moss, J., Vaughan, M.

Notes: The Transfectam® Reagent was used to transiently transfect NIH 3T3, COS-7 and HeLa cells. A lot of detail is provided for the the transfection method. Wizard® Plus Miniprep DNA Purification System and Wizard® PCR Preps DNA Purification System were used for plasmid purification and PCR product purification from low melting point agarose, respectively. (0226)

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J. Clin. Microbiol. 36, 3182-3187. Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes 1998

Wang, L., Curd, H., Qu, W., Reeves, P.R.

Notes: Chromosomal E.coli DNA was prepared with the Wizard® Genomic DNA Purification Kit and used for PCR. The resulting PCR fragments were purified with the Wizard® PCR Preps DNA Purification System and used for fluorescent sequencing. (0203)

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Genetics 148, 1539-1550. The roles of the bacteriophage T4 r genes in lysis inhibition and fine- structure genetics: a new perspective. 1998

Paddison, P., Abedon, S.T., Dressman, H.K., Gailbreath, K., Tracy, J., Mosser, E., Neitzel, J., Guttman, B., Kutter, E.

Notes: PCR products were purified by Wizard® PCR Preps DNA Purification System and sequenced with the fmol® DNA Cycle Sequencing System. (0576)

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J. Biol. Chem. 273, 14885-14890. Transcriptional regulation of endothelial nitric-oxide synthase by lysophosphatidylcholine 1998

Cieslik, K., Zembowicz, A., Tang, J.-L., Wu, K.K.

Notes: The Serine/Threonine Phosphatase Assay System was used to assess protein phosphatase 2A, 2B and 2C activity in nuclear extracts of Human umbilical vein cells (HUVEC) with or without lysophosphatidylcholine treatment. Gel shifts were also performed with nuclear extracts prepared from the HUVEC cells using the SP1 Consensus Oligonucleotide. Luciferase reporter studies were also performed in HUVEC cells using pGL3 Basic-derived vectors and the Luciferase Assay System. (1305)

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Blood 90, 669-677. A three amino acid deletion in glycoprotein IIIa is responsible for type I Glanzmann's thrombasthenia: importance of residues Ile325Pro326Gly327 for beta3 integrin subunit association. 1997

Morel-Kopp, M.C., Kaplan, C., Proulle, V., Jallu, V., Melchior, C., Peyruchaud, O., Aurousseau, M.H., Kieffer, N.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to replicate mutations identified in patients with Glanzmann's Thrombasthenia. The cDNA was cloned into the pALTER®-1 Vector and all mutation were confirmed by sequencing with the fmol® DNA Cycle Sequencing System. (0660)

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Proc. Natl. Acad. Sci. USA 94, 1069-1073. Assembly of an active enzyme by the linkage of two protein modules. 1997

Nixon, A. E. , Warren, M. S. , Benkovic, S. J.

Notes: Taq DNA Polymerase was used for PCR. The PCR products were purified with the Wizard® PCR Preps DNA Purification System and cloned with the aid of the pGEM®-T Vector System. (0624)

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J. Biol. Chem. 272, 27218-27223. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. 1997

Karlsson, M., Contreras, J.A., Hellman, U., Tornqvist, H., Holm, C.

Notes: Tryptic peptides, produced with Sequencing Grade Modified Trypsin, of the purified lipase were used to generate primers for RT-PCR. The largest amplimer was purified and used to screen a lambda gt11 library. The isolated 303 amino acid clone and site-specific mutants were put into the pCI-neo Mammalian Expression Vector and expressed in COS cells. The transiently expressed proteins were assayed for esterase and lipase activity. (0960)

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Proc. Natl. Acad. Sci. USA 94, 4211-4216. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6- desaturated fatty acids in transgenic tobacco. 1997

Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R., Napier, J.A.

Notes: The authors use the Reverse Transcription System, Wizard® PCR Preps DNA Purification Systems, Wizard® Plus Minipreps DNA Purification System and the pGEM®-T Vector System in their studies. (0445)

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Proc. Natl. Acad. Sci. USA 94, 7997-8000. Functional and nonfunctional mutations distinguished by random recombination of homologous genes 1997

Zhao, H. , Arnold, F. H.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to introduce four individual amino acid changes into the subtilisin protein. The Wizard® PCR Preps DNA Purification System was used as well. (0102)

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J. Biol. Chem. 272(32), 19827-19836. Molecular analysis of a novel winged helix protein, WIN: Expression pattern, DNA binding property and alternative splicing within the DNA binding domain. 1997

Yao, K.-M., Sha, M., Lu, Z. and Wong, G.G.

Notes: The system was used to isolate poly A+ RNA for INS-1 cell total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used in RT-PCR and Northern analysis. (1700)

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Cancer Res. 57, 2619-2622. p16INK4a promoter is hypermethylated at a high frequency in esophageal adenocarcinomas. 1997

Wong, D. J. , Barrett, M. T. , Stoger, R. , Emond, M. J. , Reid, B. J.

Notes: DNA with unmethylated cytosines converted to uracil by bisulfite treatment was purified with the Wizard® PCR Preps DNA Purification System. The DNA was used for methylation-specific PCR. (0149)

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