Lumit® TNF-α (Human) Immunoassay
No-Wash Immunoassay for TNF-α Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6050
Catalog Number: W6052
Catalog Number: W6051
Catalog Number: W137A-C
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit® TNF-α Immunoassay quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.
How the Lumit® TNF-α Immunoassay Works
Primary antibodies to TNF-α were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of TNF-α, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to TNF-α levels.
Simple One-Plate Protocol, No Wash Steps
Large Dynamic Range of Lumit® TNF-α Detection
The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.
|
Assay |
TNF-α Human Immunoassay |
|
|
Limit of Detection (LOD) |
2.9pg/ml (3 SD above background) |
|
|
Dynamic Range |
18.2pg/ml – 25ng/ml |
|
|
Minimal Detectable Dose (MDD) |
2.0pg/ml (2 SD above background) |
|
|
Assay Time |
70 minutes |
|
|
Sample Type |
Cell culture supernatants |
|
SD=Standard Deviation
Sensitive Detection of TNF-α Without Sample Dilution
When treated with a Cell Stimulation Cocktail (CSC), human PBMCs release TNF-α. After treatment for 24, 48 or 72 hours, Lumit® TNF-α Human Immunoassay reagents were added directly to cell wells to measure TNF-α release. No sample dilution was required to measure quantities of TNF-α release.
Multiple Cytokine Detection by Split-Sample Analysis
Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit® Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.
Explore Additional Cytokine Detection Assays
We offer a growing portfolio of assays to detect cytokines in cell culture samples.
Can’t find your target in the list?
Please enquire about early access to new assays in development.
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Featured Application Note
CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells
Explore a multiplexing approach that unlocks the full potential of CAR T cell therapy with our Lumit® IFN-γ and TNF-α (Human) Immunoassays.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human TNF-α Standard |
W137A | 1 × 25μl | 10μg/ml |
Anti-hTNF-α mAb-SmBiT, 1000X |
W138A | 1 × 15μl | |
Anti-hTNF-α mAb-LgBiT, 1000X |
W139A | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human TNF-α Standard |
W137A | 1 × 25μl | 10μg/ml |
Anti-hTNF-α mAb-SmBiT, 1000X |
W138A | 5 × 15μl | |
Anti-hTNF-α mAb-LgBiT, 1000X |
W139A | 5 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human TNF-α Standard |
W137A | 1 × 25μl | 10μg/ml |
Anti-hTNF-α mAb-SmBiT, 1000X |
W138B | 1 × 150μl | |
Anti-hTNF-α mAb-LgBiT, 1000X |
W139B | 1 × 150μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Human TNF-α Standard |
W137A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Articles
Other
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
- Application Note: Comparison of Lumit™ and Cisbio HTRF® Assays for Measurement of Human TNF-α
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