Direct PCR: XpressAmp® Direct Amplification Reagents
Rapid, Extraction-Free Viral Sample Preparation for RT-qPCR Amplification
- Ten-minute, room-temperature sample lysis
- Easily automatable for high-throughput needs
- Use Restrictions: For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Catalog Number:
Size
Catalog Number: A8882
Catalog Number: A8880
Direct Amplification for Rapid PCR Results
The XpressAmp® Direct Amplification Reagents provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.
How the XpressAmp® Direct Amplification Reagents Work
Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.
Lyse Sample
Mix sample and XpressAmp® Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.
Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp® Solution.
Learn how direct amplification can simplify your workflow and speed time to results for PCR or qPCR amplification and analysis. View On-Demand Webinar
Viral RNA Detection Without the Need for Extraction
XpressAmp® Detection of RNA from Inactivated Influenza/RSV Virus Pellet
Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp® Lysates
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp® Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp® Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.
Saliva Direct Protocol for SARS-CoV-2 Surveillance Testing
Direct PCR Application Notes for Extended Sample Types
Following the development of the extraction-free workflow for nasopharyngeal swabs in transport media, we continue to explore other sample applications for the XpressAmp® reagents and publish the results as short Application Notes. Explore these Application Notes to learn more about how you can use the XpressAmp® kit to prepare a variety of sample types for PCR or qPCR amplification and analysis.
Featured Application Notes:
Inactivation of SARS-CoV-2 Virus by XpressAmp® Lysis Buffer
An independent laboratory performed an evaluation of the ability of the XpressAmp® Lysis Buffer, used to prepare viral sample for RT-qPCR analysis, to inactivate the SARS-CoV-2 virus.
SARS-CoV-2 Viral Inactivation White Paper
Evidence of Viral Inactivation by XpressAmp® Lysis Buffer Video
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
XpressAmp® Lysis Buffer |
A886A | 1 × 15ml |
XpressAmp® Solution |
A887A | 1 × 1.5ml |
| 1-Thioglycerol | A208A | 2 × 75μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
XpressAmp® Lysis Buffer |
A886B | 1 × 200ml |
XpressAmp® Solution |
A887B | 1 × 18ml |
1-Thioglycerol |
A208C | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
Articles
Other
- Application Note: Direct Viral RNA Detection from XpressAmpâ„¢ Lysates
- Application Note: Direct Viral RNA Detection from Saliva using XpressAmpâ„¢ Direct Amplification Reagents
- Video: Introduction to XpressAmpâ„¢ Amplification
- Blog Post: XpressAmpâ„¢ Direct Amplification: Simplified and Accelerated Time to qPCR Results
- XpressAmpâ„¢ Direct Amplification Reagents Flyer
