ViviRen™ Live Cell Substrate
Sensitive Quantitation of <i>Renilla</i> Luminescence in Live Cells
- Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays
- Permits real-time measurement in multiwell plates
- High signal-to-background ratios
Catalog Number:
Size
Catalog Number: E6491
Catalog Number: E6492
Catalog Number: E6495
Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells
ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are each supplied as a lyophilized solid.
Assay Advantages
- Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
- Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
- Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition. CHO cells stably expressing Renilla luciferase were exposed to 20µM ViviRen™ Live Cell Substrate or coelenterazine approximately 1.5 minutes after continuous luminescent reading was initiated. For more details, see Figure 5 of TM064.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649A | 1 × 0.37mg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649B | 1 × 3.7mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
ViviRen™ Live Cell Substrate |
E649C | 1 × 37mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
No related resources available
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