Raji (LDH-HiBiT) TCK Bioassays, Propagation Model Technical Manual
Instructions for Use of Product(s)
M2432, M2042, M2062, M2072, GA6060, GA6100, GA6120, GA6130
Literature # TM737
Raji (LDH-HiBiT) TCK Bioassays, Propagation Model measure the death of Raji cells engineered to express a fusion of HiBiT to the C terminus of the lactate dehydrogenase (LDHB) gene product (LDH-HiBiT). HiBiT is an 11 amino acid peptide that binds with high affinity to LgBiT to form NanoBiT® luciferase. When dead or dying cells release the HiBiT fusion protein into the extracellular medium, it binds to the cell-impermeable LgBiT, which is provided with the furimazine substrate in a nonlytic, homogeneous detection reagent. In coculture experiments, the luminescent signal will be proportional to target cell death alone, with no signal contribution arising from the death of effector cells.
Raji (LDH-HiBiT) TCK Bioassays, Propagation Model, are a suite of four individual bioassays comprised of the parental Raji line and three different knockout (KO) variants. Each bioassay contains a clonal cell line in the cellular propagation model (CPM) and Cell Bank formats, which can be thawed, propagated and banked for long-term use. All cell lines express the LDH-HiBiT reporter, making them suitable for analysis using the HiBiT TCK technology.
The Raji (LDH-HiBiT) TCK Bioassay has been prequalified in a T cell-dependent cellular cytotoxicity (TDCC) bioassay following International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines and exhibit the precision, accuracy and linearity required for routine use in potency and stability studies. HiBiT TCK Bioassay workflows are simple, robust and compatible with both 96- and 384-well plate formats used for early biologic drug discovery and lot release settings.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Updated the Lumit trademark.
2. Revised text about the label in Section 3.
Revised 5/25.