Achieve the Protein Expression Level You Need with the Mammalian HaloTag 7 Flexi Vectors
Michael Slater, Danette Hartzell, Jim Hartnett, Sarah Wheeler, Pete Stecha, and Natasha Karassina
Promega Corporation
Publication Date: 2008
Promega Corporation
Publication Date: 2008
Abstract
A series of vectors containing deletions of human cytomegalovirus (CMV) immediate-early enhancer/promoter were created to provide different constitutive expression levels of HaloTag 7 fusion proteins, providing greater control over the level of protein expression. This allows expression of enough fusion protein to image or capture but not so much to disturb normal function of the protein partner fused to the HaloTag 7 protein.
Promega Notes 100, 16–18.
Learn about directional cloning of protein coding sequences.
