pGEM®-3Z Vector
Standard Cloning Vector that can Efficiently Synthesize RNA In vitro
- Blue/white screening to easily identify recombinant clones
- Versatile vector used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- Multiple cloning site provides a convenient selection of restriction sites for cloning
Catalog Number:
Size
Catalog Number: P2151
The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
pGEM®-3Z Vector GenBank® Accession Number X65304 and vector sequence text file.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pGEM®-3Z Vector |
P215A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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